Product Pathways - Motif Antibodies
Phospho-(Ser/Thr) PKA Substrate Antibody #9621
Looking for in-depth proteomic analysis of regulatory PTMs? PTMScan® Services are now available.
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W IP IHC-P E-P | All | Endogenous | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
Phospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. It does not cross-react with the nonphosphorylated PKA substrate motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-PKA substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) PKA Substrate Antibody.
Western Blotting
Western blot analysis of extracts from A431 cells, phosphorylated in vitro by protein kinase A, Erk2 or cdc2/cyclin A, plus or minus PKA inhibitor (PKI), using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phsopho-(Ser/Thr) PKA Substrate Antibody.
ELISA-Peptide
Phospho-(Ser/Thr) PKA Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)
Background
An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.
- Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
Application References
- Lei, H. et al. (2007) J Biol Chem 282, 9364-71. Applications: Western Blotting
- Marcinkiewicz, A. et al. (2006) J Biol Chem 281, 11901-9. Applications: Western Blotting
- Su, Y.H. and Vacquier, V.D. (2006) Mol Biol Cell 17, 114-21. Applications: Western Blotting
- Miyoshi, H. et al. (2006) J Biol Chem 281, 15837-44. Applications: Western Blotting
- Smith, J.A. et al. (2005) Cancer Res 65, 1027-34. Applications: Western Blotting
- Wada, S. et al. (2005) J Biol Chem 280, 94-103. Applications: Western Blotting
- Hatakeyama, M. et al. (2004) Oncogene 23, 5023-31. Applications: Western Blotting
- Schmitt, J.M. and Stork, P.J. (2002) Mol Cell 9, 85-94. Applications: Western Blotting
- Grønborg, M. et al. (2002) Mol Cell Proteomics 1, 517-27. Applications: IP Western Blotting
- Bruce, J.I. et al. (2002) J Biol Chem 277, 1340-8. Applications: IP Western Blotting
- Schmitt, A. and Nebreda, A.R. (2002) Proc Natl Acad Sci U S A 99, 4361-6. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9624 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
- 9614 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb
- 2261 Phospho-(Ser) PKC Substrate Antibody
- 2321 Phospho-(Thr) MAPK/CDK Substrate Mouse mAb
- 2351 Phospho-Threonine-X-Arginine Antibody
- 9381 Phospho-Threonine Antibody (P-Thr-Polyclonal)
- 9391 Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)
- 9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody
- 9611 Phospho-(Ser/Thr) Akt Substrate Antibody
- 9631 Phospho-(Ser/Thr) Phe Antibody
- 9386 Phospho-Threonine (42H4) Mouse mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
