Product Pathways - Motif Antibodies
Phospho-(Ser/Thr) PKA Substrate Antibody #9621
| Applications | Reactivity | Source |
|---|---|---|
| W IP IHC-P E-P | All | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Specificity / Sensitivity
Phospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. It does not cross-react with the nonphosphorylated PKA substrate motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-PKA substrate peptide (KLH-coulpled). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) PKA Substrate Antibody.
Western Blotting
Western blot analysis of extracts from A431 cells, phosphorylated in vitro by protein kinase A, Erk2 or cdc2/cyclin A, plus or minus PKA inhibitor (PKI), using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phsopho-(Ser/Thr) PKA Substrate Antibody.
ELISA-Peptide
Phospho-(Ser/Thr) PKA Substrate Antibody DELFIA® Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) (DELFIA® is a registered trademark of PerkinElmer, Inc.)
Background
An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.
- Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
Application References
- Schmitt, J.M. and Stork, P.J. (2002) PKA phosphorylation of Src mediates cAMP's inhibition of cell growth via Rap1. Mol. Cell 9, 85-94. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting
- Hatakeyama, M. et al. (2004) Transformation potency of ErbB heterodimer signaling is determined by B-Raf kinase. Oncogene 23, 5023-5031. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting
- Wada, S. et al. (2005) Phosphorylation of candida glabrata ATP-binding cassette transporter Cdr1p regulates drug efflux activity and ATPase stability. J. Biol. Chem. 280, 94-103. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting
- Gronborg, M. et al. (2002) A mass spectrometry-based proteomic approach for identification of serine/threonine-phosphorylated proteins by enrichment with phospho-specific antibodies: identification of a novel protein, Frigg, as a protein kinase A substrate. Mol. Cell. Proteomics 1, 517-527. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: IP Western Blotting
- Bruce, J. I. et al. (2002) Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. J. Biol. Chem. 277 (2), 1340-1348. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: IP Western Blotting
- Schmitt, A. and Nebreda, A.R. (2002) Inhibition of Xenopus oocyte meiotic maturation by catalytically inactive protein kinase A. Proc. Natl. Acad. Sci. USA 99 (7), 4361-4366. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting
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License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.