Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) PKA Substrate Antibody #9621

• pathway
• more info
• application references
• datasheet PDF
• MSDS PDF
• protocols

Applications	Reactivity	Source
W IP IHC-P E-P	All	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  E-P=ELISA (Peptide)
Reactivity Key: All=All species expected

Specificity / Sensitivity

Phospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. It does not cross-react with the nonphosphorylated PKA substrate motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-PKA substrate peptide (KLH-coulpled). Antibodies are purified by protein A and peptide affinity chromatography.

Background

An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.

Application References

• Schmitt, J.M. and Stork, P.J. (2002) PKA phosphorylation of Src mediates cAMP's inhibition of cell growth via Rap1. Mol. Cell 9, 85-94. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting
• Hatakeyama, M. et al. (2004) Transformation potency of ErbB heterodimer signaling is determined by B-Raf kinase. Oncogene 23, 5023-5031. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting
• Wada, S. et al. (2005) Phosphorylation of candida glabrata ATP-binding cassette transporter Cdr1p regulates drug efflux activity and ATPase stability. J. Biol. Chem. 280, 94-103. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting
• Gronborg, M. et al. (2002) A mass spectrometry-based proteomic approach for identification of serine/threonine-phosphorylated proteins by enrichment with phospho-specific antibodies: identification of a novel protein, Frigg, as a protein kinase A substrate. Mol. Cell. Proteomics 1, 517-527. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: IP Western Blotting
• Bruce, J. I. et al. (2002) Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. J. Biol. Chem. 277 (2), 1340-1348. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: IP Western Blotting
• Schmitt, A. and Nebreda, A.R. (2002) Inhibition of Xenopus oocyte meiotic maturation by catalytically inactive protein kinase A. Proc. Natl. Acad. Sci. USA 99 (7), 4361-4366. This article references the use of Phospho-(Ser/Thr) PKA Substrate Antibody in the following applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9624 Phospho-PKA Substrate (RRXS/T) (100G7E) Rabbit mAb
• 9614 Phospho-Akt Substrate (RXRXXS/T) (110B7E) Rabbit mAb
• 2324 Phospho-(Ser) CDKs Substrate Antibody
• 2261 Phospho-(Ser) PKC Substrate Antibody
• 2321 Phospho-(Thr) MAPK/CDK Substrate Mouse mAb
• 2351 Phospho-Threonine-X-Arginine Antibody
• 9381 Phospho-Threonine Antibody (P-Thr-Polyclonal)
• 9391 Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)
• 9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody
• 9611 Phospho-(Ser/Thr) Akt Substrate Antibody
• 9631 Phospho-(Ser/Thr) Phe Antibody
• 9386 Phospho-Threonine (42H4) Mouse mAb
• 9920 Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.