Cell Signaling Technology

Product Pathways - Apoptosis

Lamin B2 Antibody #9622

Applications Reactivity Sensitivity MW (kDa) Source
W H M (Mk) (Pg) Endogenous 68 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Lamin B2 Antibody recognizes endogenous levels of total lamin B2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu75 of human lamin B2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lamin B2 Antibody.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, arising from alternative splicing. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene is correlated with the pathogenesis of the neurological disorder adult-onset leukodystrophy (10).

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  3. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  4. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
  5. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  6. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
  7. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
  8. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
  9. Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
  10. Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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