Product Pathways - Neuroscience
Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | M R Dm (H) | Endogenous | 50-80 | Mouse IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Tau (Ser396) (PHF13) Mouse mAb detects endogenous levels of Tau only when phosphorylated at serine 396.
Source / Purification
Monoclonal antibody is produced by immunizing mice with human purified Tau (Hoffmann et al., 1997).
Western Blotting
Western blot analysis of extracts from mouse brain and CAD cells, using Phospho-Tau (Ser396) (PHF13) Mouse mAb. The phospho-specificity of the antibody was verified by peptide blocking using no peptide, phospho-peptide or nonphospho-peptide.
Background
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
- Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
- Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
- Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
Application References
- Hoffmann, R. et al. (1997) Biochemistry 36, 8114-8124. This article references the use of Phospho-Tau (Ser396) (PHF13) Mouse mAb in the following applications: Western Blotting
- Hong, M. and Lee, V.M. (1997) J. Biol. Chem. 272, 19547-19553. This article references the use of Phospho-Tau (Ser396) (PHF13) Mouse mAb in the following applications: Western Blotting
- Hong, M. et al. (1997) J. Biol. Chem. 272, 25326-25332. This article references the use of Phospho-Tau (Ser396) (PHF13) Mouse mAb in the following applications: Western Blotting
- Ahlijanian, M.K. et al. (2000) Proc Natl Acad Sci U S A 97, 2910-2915. This article references the use of Phospho-Tau (Ser396) (PHF13) Mouse mAb in the following applications: IHC-P (paraffin) Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
