Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP M R Dm (H) Endogenous 50-80 Mouse IgG2b

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Tau (Ser396) (PHF13) Mouse mAb detects endogenous levels of Tau only when phosphorylated at serine 396.

Source / Purification

Monoclonal antibody is produced by immunizing mice with human purified Tau (Hoffmann et al., 1997).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and CAD cells, using Phospho-Tau (Ser396) (PHF13) Mouse mAb. The phospho-specificity of the antibody was verified by peptide blocking using no peptide, phospho-peptide or nonphospho-peptide.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

  1. Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
  2. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
  3. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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