Product Pathways - Neuroscience
Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632
PhosphoSitePlus® protein, site, and accession data: tau iso8
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | M R (H) | Endogenous | 50-80 | Mouse IgG2b |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9632:
- Western Blotting
Specificity / Sensitivity
Phospho-Tau (Ser396) (PHF13) Mouse mAb detects endogenous levels of Tau only when phosphorylated at serine 396.
Source / Purification
Monoclonal antibody is produced by immunizing animals with human purified Tau (Hoffmann et al., 1997).
Western Blotting
Western blot analysis of extracts from mouse brain and CAD cells, using Phospho-Tau (Ser396) (PHF13) Mouse mAb. The phospho-specificity of the antibody was verified by peptide blocking using no peptide, phospho-peptide or nonphospho-peptide.
Background
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
- Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
- Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
- Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
Application References
- Hoffmann, R. et al. (1997) Biochemistry 36, 8114-8124. Applications: Western Blotting
- Hong, M. and Lee, V.M. (1997) J. Biol. Chem. 272, 19547-19553. Applications: Western Blotting
- Hong, M. et al. (1997) J. Biol. Chem. 272, 25326-25332. Applications: Western Blotting
- Ahlijanian, M.K. et al. (2000) Proc Natl Acad Sci U S A 97, 2910-2915. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
