Product Pathways -
Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 555 Conjugate) #9641
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | Endogenous | Mouse IgG1 |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9641:
- Immunofluorescence
Specificity / Sensitivity
Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 555 Conjugate) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.
Source / Purification
IF-IC
Confocal immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red, left) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 555 Conjugate) (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology (CST) antibody is conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and tested in-house for direct immunofluorescent analysis in human cells.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
