Cell Signaling Technology

Product Pathways - Metabolism

PFKFB3 Antibody #9645

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 60 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PFKFB3 Antibody recognizes endogenous levels of total PFKFB3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly452 of human PFKFB3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PFKFB3 Antibody.

Background

The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). One of them, PFKFB3/iPFK-2, was shown to be inducible by hypoxia leading to increased glycolysis under hypoxic conditions (2). Research studies have shown that PFKFB3/iPFK-2 is also highly expressed in some types of human cancer (3).

  1. Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
  2. Kim, S.G. et al. (2006) J Biol Chem 281, 2939-44.
  3. Atsumi, T. et al. (2002) Cancer Res 62, 5881-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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