Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

RUNX3/AML2 (D6E2) Rabbit mAb #9647

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 43-48 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RUNX3/AML2 (D6E2) Rabbit mAb recognizes endogenous levels of both isoforms of RUNX3 protein. This antibody does not recognize RUNX1 and RUNX2 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RUNX3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SW620 and HeLa cells using RUNX3/AML2 (D6E2) Rabbit mAb.

Background

RUNX3/AML2 is a member of the Runt family of transcription factors. RUNX3 is important for the suppression of cell proliferation in the gastric epithelium (1), neurogenesis of the dorsal root ganglia (2), and T cell differentiation (3,4). According to the research literature, RUNX3 is found to be inactivated in more than 80% of gastric cancers and other cancer types by gene silencing or protein mislocalization (1,5,6). The tumor suppressor function of RUNX3 is exerted by forming complexes with various transcription factors, such as Smads or β-Catenin/TCF4 to regulate downstream target gene transcription (7,8). RUNX3 is also involved in caspase-3-dependent apoptosis (9). RUNX3 is normally located in the nucleus, however, in many cancer cells, RUNX3 is tyrosine phosphorylated and mislocated to the cytoplasm. The mislocation of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (10).

  1. Li, Q.L. et al. (2002) Cell 109, 113-24.
  2. Inoue, K. et al. (2002) Nat Neurosci 5, 946-54.
  3. Taniuchi, I. et al. (2002) Cell 111, 621-33.
  4. Woolf, E. et al. (2007) Dev Biol 303, 703-14.
  5. Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
  6. Ito, K. et al. (2005) Cancer Res 65, 7743-50.
  7. Chi, X.Z. et al. (2005) Mol Cell Biol 25, 8097-107.
  8. Ito, K. et al. (2008) Cancer Cell 14, 226-37.
  9. Zhai, F.X. et al. (2012) J Cancer Res Clin Oncol 138, 439-49.
  10. Goh, Y.M. et al. (2010) J Biol Chem 285, 10122-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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