Product Pathways - TGF-beta/Smad Signaling
RUNX3/AML2 (D6E2) Rabbit mAb #9647
|9647S||100 µl (10 western blots)||---||In Stock||---|
|9647||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||43-48||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
RUNX3/AML2 (D6E2) Rabbit mAb recognizes endogenous levels of both isoforms of RUNX3 protein. This antibody does not recognize RUNX1 and RUNX2 proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RUNX3 protein.
Runt-related transcription factor 3 (RUNX3, AML2), a member of the Runt family of transcription factors, plays an important role in the suppression of gastric epithelium cell proliferation (1), dorsal root ganglia neurogenesis (2), and T cell differentiation (3,4). RUNX3 is also involved in caspase-3-dependent apoptosis (5). Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin/TCF4, have tumor suppressor activity and regulate downstream target gene transcription (6,7). While typically localized to the nucleus, RUNX3 can be tyrosine phosphorylated and located in the cytoplasm of many cancer cells. This mislocalization of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (8). Research studies indicate that gene silencing or protein mislocalization inactivates RUNX3 in more than 80% of gastric cancers and other cancer types (1,9,10).
- Li, Q.L. et al. (2002) Cell 109, 113-24.
- Inoue, K. et al. (2002) Nat Neurosci 5, 946-54.
- Taniuchi, I. et al. (2002) Cell 111, 621-33.
- Woolf, E. et al. (2007) Dev Biol 303, 703-14.
- Zhai, F.X. et al. (2012) J Cancer Res Clin Oncol 138, 439-49.
- Chi, X.Z. et al. (2005) Mol Cell Biol 25, 8097-107.
- Ito, K. et al. (2008) Cancer Cell 14, 226-37.
- Goh, Y.M. et al. (2010) J Biol Chem 285, 10122-9.
- Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
- Ito, K. et al. (2005) Cancer Res 65, 7743-50.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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