Product Pathways - PI3K / Akt Signaling
PTEN and PDK1 Antibody Sampler Kit #9652
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549||40 µl||W||H M R Mk||54||Rabbit IgG|
|PTEN (D4.3) XP® Rabbit mAb #9188||40 µl||W IP IHC-P||H M R Mk Dg (C)||54||Rabbit IgG|
|Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb #3438||40 µl||W IP||H M R||58 to 68||Rabbit IgG|
|PDK1 Antibody #3062||40 µl||W||H M R Mk||58 to 68||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) Rabbit mAb #7960||40 µl||W IP||H M R Mk||55||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the PTEN and PDK1 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. Non-Phospho-PTEN (Ser380/Thr382/Thr383) Antibody detects endogenous levels of PTEN only when dephosphorylated at Ser380, Thr382 and Thr383.
Western blot analysis of extracts from SW-13, NIH/3T3, Jurkat and PC-12 cells using PDK1 Antibody #3062.
Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb #3438 (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower).
Western blot analysis of extracts from various cell lines using Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) Rabbit mAb #7960. The non-phosphospecificity of the antibody was verified by preincubating the antibody without peptide (-), with PTEN (Ser380/Thr382/Thr383) non-phosphopeptide (+), or with PTEN (Ser380/Thr382/Thr383) phosphopeptide (+) prior to incubating the membrane.
Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP® Rabbit mAb #9188 (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).
Western blot analysis of extracts from HeLa and NIH/3T3 cells using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549. Membranes were left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) following western transfer to verify phospho-specificity of the antibody.
The PTEN and PDK1 Sampler Kit provides an economical means to evaluate two key enzymes that regulate multiple signaling pathways. The kit contains enough primary and secondary antibodies to perform four Western blots per primary antibody.
Source / Purification
Phospho-specific rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN and around Ser241 of human PDK1. PTEN (D4.3) XP® Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of human PTEN. Monoclonal antibody Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) is produced by immunizing animals with a synthetic non-phosphopeptide corresponding to residues surrounding Ser380/Thr382/Thr383 of human PTEN protein. Polyclonal PDK1 antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding the carboxy terminus of human PDK1. Polyclonal antibodies are purified using protein A and peptide affinity chromatography.
PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (11,12) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (13). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.
- Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
- Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
- Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
- Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
- Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
- Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
- Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
- Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
- Funamoto, S. et al. (2002) Cell 109, 611-23.
- Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
- Belham, C. et al. (1999) Curr Biol 9, R93-6.
- Toker, A. and Newton, A.C. (2000) Cell 103, 185-8.
- Williams, M.R. et al. (2000) Curr Biol 10, 439-48.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7960 Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) Rabbit mAb
Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and/or U.S.S.N. 11/476,277) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.