Product Pathways - PI3K / Akt Signaling
PI3 Kinase Antibody Sampler Kit #9655
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody #4228 | 40 µl | W IP | M (H) (R) (Mk) (B) | 60 and 85 | Rabbit |
| PI3 Kinase p85 (19H8) Rabbit mAb #4257 | 40 µl | W IP | H M R (C) | 85 | Rabbit IgG |
| PI3 Kinase p110α (C73F8) Rabbit mAb #4249 | 40 µl | W IP IHC-P | H M R B | 110 | Rabbit IgG |
| PI3 Kinase p110β (C33D4) Rabbit mAb #3011 | 40 µl | W IP | H | 110 | Rabbit IgG |
| PI3 Kinase Class III (D4E2) Rabbit mAb #3358 | 40 µl | W | H M R Mk | 100 | Rabbit IgG |
| PI3 Kinase p110γ (D55D5) Rabbit mAb #5405 | 40 µl | W IP | H M (R) | 110 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the PI3 Kinase Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each total antibody detects endogenous levels of its target protein. Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody detects endogenous levels of p85/p55 only when phosphorylated at Tyr458/Tyr199.
Western Blotting
Western blot analysis of extracts from MCF-7 and K-562 cells using PI3 Kinase p110β (C33D4) Rabbit mAb #3011.
Western Blotting
Western blot analysis of extracts from 293 and RN33B cells using PI3 Kinase Class III (D4E2) Rabbit mAb #3358.
Western Blotting
Western blot analysis of extracts from NIH/3T3-Src cells, untreated or λ-phosphatase-treated, and from C2C12 cells, untreated or H2O2-treated, using Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody #4228.
Western Blotting
Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb #4249.
Western Blotting
Western blot analysis of extracts from Jurkat and NIH/3T3 cells using PI3 Kinase p85 (19H8) Rabbit mAb #4257.
Western Blotting
Western blot analysis of extracts from NIH/3T3, Jurkat and K562 cells using PI3 Kinase p110-γ (D55D5) Rabbit mAb #5405.
Description
The PI3 Kinase Sampler Kit provides an economical means of studying PI3 kinase subunits in cells. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
Source / Purification
Phospho-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr458 of mouse p85. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human PI3K p85, corresponding to human PI3K p110-gamma, surrounding Asp520 from human PI3K p110α, containing human PI3K p110β, and the amino-terminal sequence of human PI3K class III.
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
- Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
- Stoyanov, B. et al. (1995) Science 269, 690-3.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 3011:
- Immunoprecipitation, Western Blotting
- 3358:
- Western Blotting
- 4228:
- Immunoprecipitation, Western Blotting
- 4249:
- IHC / Paraffin, Immunoprecipitation, Western Blotting
- 4257:
- Immunoprecipitation, Western Blotting
- 5405:
- Immunoprecipitation, Western Blotting
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and/or U.S.S.N. 11/476,277) from Epitomics, Inc.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
