Product Pathways - Apoptosis / Autophagy
Caspase-3 Antibody #9662
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P | H M R | Endogenous | 17, 19, 35 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding the cleavage site of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated, and NIH/3T3 cells, untreated or staurosporine-treated, using Caspase-3 Antibody.
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human tonsil, showing cytoplasmic localization, using Caspase-3 Antibody.
Background
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
- Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
Application References
- Erhardt, J. A. et al. (2001) Activation of caspase-3/caspase-3-like activity in rat cardiomyocytes by an RGD peptide, but not the GPIIb/IIIa antagonist lotrafiban. Thromb. Res. 103, 143-148. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
- Marques, C. A. et al. (2003) Neurotoxic mechanisms caused by the Alzheimer's disease-linked Swedish amyloid precursor protein mutation:oxidative stress, caspases, and the JNK pathway. J Biol Chem 278 (30), 28294-302. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
- Jeong, W. et al. (2004) Roles of TRP14, a thioredoxin-related protein in tumor necrosis factor-alpha signaling pathways. J Biol Chem. 279 (5), 3151-9. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
- Ballestrero, A. et al. (2004) Tumor necrosis factor-related apoptosis-inducing ligand cooperates with anticancer drugs to overcome chemoresistance in antiapoptotic Bcl-2 family members-expressing Jurkats cells. Clin Cancer Res 10 (5), 1463-70. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
- Chandrasekar, B. et al. (2004) Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death. J. Biol. Chem. 279, 20221-20233. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
- Nishihara, H. et al. (2003) Inhibition of apoptosis in normal and transformed intestinal epithelial cells by cAMP through induction of inhibitor of apoptosis protein (IAP)-2. PNAS 100 (15), 8921-8926. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
- Yu, L. et al. (2002) TGF-beta receptor-activated p38 MAP kinase mediates Smad-independent TGF-beta responses. EMBO J. 21 (14), 3749-3759. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
- Hara, H. et al. (2002) The apoptotic protease-activating factor 1-mediated pathway of apoptosis is dispensable for negative selection of thymocytes. J. Immunol. 168, 2288-2295. This article references the use of Caspase-3 Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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- 9748 Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
