Cell Signaling Technology

Product Pathways - Apoptosis

Caspase-3 Control Cell Extracts #9663

Description

Untreated Jurkat Control Cell Extracts: Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated to serve as a negative control for caspase cleavage. Cytochrome c Treated Jurkat Cell Extracts: Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated. Extracts are then treated with cytochrome c in vitro to generate a positive control for caspase cleavage.

Applications

Western Blots: CST recommends boiling control cell extracts for two minutes prior to loading. Load 10 µl of each Jurkat control cell extract per lane.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

  1. Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764.
  2. Nicholson, D. W. et al. (1995) Nature 376, 37-43.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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