Product Pathways - Apoptosis / Autophagy
Caspase-3 (8G10) Rabbit mAb #9665
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP | H M R | Endogenous | 17, 19, 35 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Caspase-3 (8G10) Rabbit mAb detects endogenous levels of full-length (35 kDa) and large fragment (17/19 kDa) of caspase-3 resulting from cleavage at aspartic acid 175.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3.
Western Blotting
Western analysis of HeLa (human), NIH/3T3 (mouse) cell extracts treated with 1 uM staurosporine in vivo using Caspase-3 (8G10) Rabbit mAb.
IP
Immunoprecipitation of cleaved caspase-3 from Jurkat cell extracts untreated (control) or treated with etoposide (apoptotic) using Caspase-3 (8G10) Rabbit mAb, and western probed with the same antibody.
Background
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).
- Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
