Cell Signaling Technology

Product Pathways - Transcription Factors

PAX2 Antibody #9666

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R (Mk) Endogenous 47 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PAX2 Antibody recognizes endogenous levels of total PAX2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His282 of human PAX2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat fetal kidney tissues using PAX2 Antibody.

Background

Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).

PAX2 is involved in the development of the nervous and excretory systems including the kidney and urogentical tract, the optic stalk, ear, midbrain-hindbrain junction, and the spinal cord (5,6).

  1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
  2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
  3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
  4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
  5. Schedl, A. and Hastie, N.D. (2000) Curr Opin Genet Dev 10, 543-9.
  6. Torres, M. et al. (1995) Development 121, 4057-65.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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