Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Caspase-3 (3G2) Mouse mAb #9668

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 17, 19, 35 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Caspase-3 (3G2) Mouse mAb detects endogenous levels of full length (35 kDa) and the large fragment (17/19 kDa) of caspase-3 resulting from cleavage at aspartic acid 175.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids 28-44 of human caspase-3.

Western Blotting

Western Blotting

Western blot analysis of HeLa cell extracts treated with 1uM staurosporine (3hrs), using Caspase-3 (3G2) Mouse mAb.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

  1. Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764.
  2. Nicholson, D. W. et al. (1995) Nature 376, 37-43.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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