Product Pathways - Chromatin Regulation / Epigenetics
Acetyl-Histone H3 (Lys18) Antibody #9675
|9675S||100 µl (10 western blots)||---||In Stock||---|
|9675P||40 µl (4 western blots)||---||In Stock||---|
|9675||carrier free and custom formulation / quantity||email request|
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|W||1:2000||Human, Mouse, Rat||Endogenous||17||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP
Specificity / Sensitivity
Acetyl-Histone H3 (Lys18) Antibody detects endogenous levels of histone H3 only when acetylated at Lys18. It does not cross-react with other acetylated histones.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from NIH/3T3 cells, untreated or TSA-treated, using Acetyl-Histone H3 (Lys18) Antibody.
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder showing nuclear localization using Acetyl-Histone H3 (Lys18) Antibody.
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or TSA-treated (right), using Acetyl-Histone H3 (Lys18) Antibody. (no counterstain)
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 20 μl of Acetyl-Histone H3 (Lys18) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
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- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
- Cheung, P. et al. (2000) Cell 103, 263-71.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
- Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
- Dai, J. et al. (2005) Genes Dev 19, 472-88.
- Dai, B. and Rasmussen, T.P. (2007) Stem Cells 25, 2567-74.
- Allison, S.J. and Milner, J. (2003) Cancer Res. 63, 6674-6679. Applications: Western Blotting.
- Parsons, X. H. et al. (2003) Proc. Nat. Acad. Sci. USA 100, 1609-1614. Applications: Western Blotting.
- Song, N. et al. (2011) Acta Histochem Cytochem 44, 183-90. Applications: IHC-P (paraffin).
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