Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Sec24A Antibody #9678

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 120 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Sec24A Antibody detects endogenous levels of total Sec24A protein. This antibody does not cross-react with other members of Sec24 family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe52 of human Sec24A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Sec24A Antibody.

Background

Coat Protein Complex II (COPII) is composed of five cytosolic proteins: Sec23/24 complex, Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex, thereby forming a prebudding complex that directly binds target molecules (1-3). The prebudding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec24 subunit of COPII coat is thought to play a critical role in cargo selection (2,6). It binds directly to cargo proteins at the ER and brings them to COPII vesicles through interaction with Sec23. There are four Sec24 isoforms in human cells: Sec24A, Sec24B, Sec24C, and Sec24D (7). In mice, mutations in Sec24B have been linked to developmental defects (8,9).

  1. Aridor, M. et al. (1998) J Cell Biol 141, 61-70.
  2. Miller, E.A. et al. (2003) Cell 114, 497-509.
  3. Mossessova, E. et al. (2003) Cell 114, 483-95.
  4. Barlowe, C. et al. (1994) Cell 77, 895-907.
  5. Bi, X. et al. (2007) Dev Cell 13, 635-45.
  6. Miller, E. et al. (2002) EMBO J 21, 6105-13.
  7. Tang, B.L. et al. (1999) Biochem Biophys Res Commun 258, 679-84.
  8. Merte, J. et al. (2010) Nat Cell Biol 12, 41-6; sup pp 1-8.
  9. Wansleeben, C. et al. (2010) Development 137, 1067-73.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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