Cell Signaling Technology

Product Pathways - Neuroscience

NT5E (CD73) Antibody #9684

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) Endogenous 70 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NT5E (CD73) Antibody recognizes endogenous levels of total NT5E protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp522 of human NT5E protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with cobalt chloride (0.1 mM, 16 hr; +), using NT5E (CD73) Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 and Huh7 cells, and rat eye using NT5E (CD73) Antibody.

Background

Ecto-5'nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleoside (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine-monophosphate, therefore driving the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promotor leads to upregulation of NT5E during hypoxia (3). NT5E biological roles span from lymphocyte adhesion (4,5), to fibrosis (6), to regulation of nociception (7,8).

  1. Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.
  2. Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.
  3. Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.
  4. Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.
  5. Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.
  6. Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.
  7. Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.
  8. Zylka, M.J. (2011) Trends Mol Med 17, 188-96.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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