Product Pathways - Motif Antibodies
Nitro-Tyrosine Antibody #9691
| Applications | Reactivity | Source |
|---|---|---|
| W | All | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key: All=All species expected
Specificity / Sensitivity
Nitro-Tyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. The antibody does not cross-react with unmodified tyrosine or with phospho-tyrosine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with synthetic nitro-tyrosine-containing peptides (KLH-coupled). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, untreated or treated with peroxynitrite, degraded peroxynitrite or pervanadate, using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (upper) or Nitro-Tyrosine Antibody (lower).
Background
Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2) and neurodegeneration (3). High levels of both superoxide and nitric oxide in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).
- Bentz, B. G. et al. (2000) Head Neck 22, 64-70.
- Jaiswal, M. et al. (2000) Cancer Res. 60, 184-190.
- Olivenza, R. et al. (2000) J. Neurochem. 74, 785-791.
- MacMillan-Crow, L. A. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 11853-11858.
- Chazotte-Aubert, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 609-613.
Application References
- Sorensen, L. et al. (2001) Late-onset corticohippocampal neurodepletion attributable to catastrophic failure of oxidative phosphorylation in MILON mice. J. Neurosci. 21 (20), 8082-8090. This article references the use of Nitro-Tyrosine Antibody in the following applications: IHC-FL (floating/frozen) Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100)
- 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.
