Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PIP5K1A Antibody #9693

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 62 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PIP5K1A Antibody detects endogenous levels of total PIP5K1A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Gly64 of human PIP5K1A. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PIP5K1A Antibody.

Background

Phosphatidylinositol-5-phosphate 4-kinases (PIP4K) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).

  1. Rameh, L.E. et al. (1997) Nature 390, 192-6.
  2. Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
  3. Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
  4. Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
  5. Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
  6. Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
  7. Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
  8. Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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