Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PIP4K2B Antibody #9694

Applications Reactivity Sensitivity MW (kDa) Source
W F H M R Mk Endogenous 48 Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PIP4K2B Antibody recognizes endogenous levels of total PIP4K2B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues of human PIP4K2B. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PIP4K2B Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using PIP4K2B Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Phosphatidylinositol-5-phosphate 4-kinases (PIP4K) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).

  1. Rameh, L.E. et al. (1997) Nature 390, 192-6.
  2. Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
  3. Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
  4. Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
  5. Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
  6. Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
  7. Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
  8. Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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