Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PIP5K2B Antibody #9694

Applications Reactivity Sensitivity MW (kDa) Source
W F H M R Mk Endogenous 48 Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PIP5K2B Antibody detects endogenous levels of total PIP5K2B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues of human PIP5K2B. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PIP5K2B Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using PIP5K2B Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Phosphatidylinositol-4-phosphate 5-kinases (PIP5K) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that also regulates some proteins and cellular processes directly. There are two subfamilies of PIP5Ks, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP5K1s use PtdIns4P as a substrate, whereas PIP5K2s use PtdIns5P (1,2). In mammalian cells, three isoforms of each PIP5K1 and PIP5K2 subfamily, encoded by distinct genes, have been characterized and named α, β and γ (3-7), and additional PIPKs likely remain to be discovered. All PIP5K isoforms are stimulated by phosphatidic acid, are extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).

  1. Rameh, L.E. et al. (1997) Nature 390, 192-6.
  2. Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
  3. Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
  4. Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
  5. Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
  6. Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
  7. Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
  8. Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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