Cell Signaling Technology

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DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 594 Conjugate) #9696

Applications Reactivity Sensitivity Source
IF-IC All Transfected Only Rabbit

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 594 Conjugate) detects exogenously expressed DYKDDDDK-tagged proteins in cells. The antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide.

IF-IC

IF-IC

Confocal immunofluorescent analysis of COS-7 cells, transiently transfected with a DYKDDDDK-tagged fusion protein, using DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in cells transfected with DYKDDDDK-tagged protein. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).

  1. Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.

Application References

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Companion Products

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.Anti-FLAG® is a registered trademark of Sigma-Aldrich.

For Research Use Only. Not For Use In Diagnostic Procedures.

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