Product Pathways - Translational Control
Phospho-eIF2α (Ser51) Antibody #9721
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk Sc Dm | Endogenous | 38 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Sc=S. cerevisiae
Dm=D. melanogaster
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha Antibody #9722 (lower).
Background
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
- Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
- De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
- Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
- Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
- Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.
Application References
- Williams, D. D. et al. (2000) Characterization of the initiation factor eIF2B and its regulation in Drosophila melanogaster. J. Biol. Chem. 276, 3733-3742. This article references the use of Phospho-eIF2α (Ser51) Antibody in the following applications: Western Blot
- Koumenis, C. et al. (2002) Regulation of protein synthesis by hypoxia via activation of the endoplasmic reticulum kinase PERK and phosphorylation of the translation initiation factor eIF2alpha. Mol Cell Biol 22, 7405-7416. This article references the use of Phospho-eIF2α (Ser51) Antibody in the following applications: Western Blotting
- Li, W. et al. (2004) Mutations linked to leukoencephalopathy with vanishing white matter impair the function of the eukaryotic initiation factor 2B complex in diverse ways. Mol. Cell. Biol. 24, 3295-3306. This article references the use of Phospho-eIF2α (Ser51) Antibody in the following applications: Western Blotting
- Connor, J. H. et al. (2001) Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1. Mol. Cell. Biol. 21, 6841-6850. This article references the use of Phospho-eIF2α (Ser51) Antibody in the following applications: Western Blot
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Companion Products
- 3597 Phospho-eIF2α (Ser51) (119A11) Rabbit mAb
- 2103 eIF2α (L57A5) Mouse mAb
- 9722 eIF2α Antibody
- 3595 eIF2B-ε Antibody
- 3302 GCN2 Antibody
- 1221 Phospho-eIF2α (Ser51) Blocking Peptide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
