Product Pathways - Translational Control
eIF2α Antibody #9722
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 38 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eIF2α Antibody detects endogenous levels of total eIF2α protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the carboxy-terminal sequence of eIF2α. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM) using Phospho-eIF2α (Ser51) Antibody #9721 (upper) and control eIF2α Antibody (lower).
Background
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
- Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
- De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
- Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
- Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
- Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.
Application References
- Nanbo, A. et al. (2002) Epstein-Barr virus RNA confers resistance to interferon-alpha-induced apoptosis in Burkitt's lymphoma. EMBO J. 21, 954-965. This article references the use of eIF2α Antibody in the following applications: Western Blotting
- Koumenis, C. et al. (2002) Regulation of protein synthesis by hypoxia via activation of the endoplasmic reticulum kinase PERK and phosphorylation of the translation initiation factor eIF2alpha. Mol. Cell. Biol. 22, 7405-7416. This article references the use of eIF2α Antibody in the following applications: Western Blotting
- Chang, R. C. et al. (2002) Involvement of double-stranded RNA-dependent protein kinase and phosphorylation of eukaryotic initiation factor-2alpha in neuronal degeneration. J. Neurochem. 83, 1215-1225. This article references the use of eIF2α Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3597 Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb
- 9721 Phospho-eIF2alpha (Ser51) Antibody
- 3302 GCN2 Antibody
- 3595 eIF2B-ε Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
