Product Pathways - Tyrosine Kinase / Adaptors
FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740
|9740S||100 µl (10 western blots)||---||In Stock||---|
|9740P||40 µl (4 western blots)||---||In Stock||---|
|9740||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||92 , 120, 145||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
FGF Receptor 1 (D8E4) XP® Rabbit mAb detects endogenous levels of total FGF receptor 1 protein. This antibody may slightly cross-react with overexpressed FGF receptor family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.
Western blot analysis of extracts from A-204 (FGFR1 positive), KG-1a (FGFR1 oncogenic partner-FGFR1 fusion), A172 (FGFR1 low), and HT-29 (FGFR1 negative) cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of A-204 cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human kidney using FGF Receptor 1 (D8E4) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.
Confocal immunofluorescent analysis of A204 cells (positive, left), KG-1 cells (positive, middle) and A172 cells (weak expression, right) using FGF Receptor 1 (D8E4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
SignalStain® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.