Product Pathways - Translational Control
Phospho-eIF4E (Ser209) Antibody #9741
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 25 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at serine 209.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser209 of human eIF4E. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody (upper) or eIF4E Antibody #9742 (lower).
Background
Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.
- Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
- Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
- Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
- Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
- Raught, B. et al. (2000) EMBO J. 19, 434-444.
Application References
- He, Y. et al. (2001) Regulation of mRNA translation and cellular signaling by hepatitis C virus nonstructural protein NS5A. J. Virol. 75, 5090-5098. This article references the use of Phospho-eIF4E (Ser209) Antibody in the following applications: Western Blot
- Banerjee, S. et al. (2002) Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937-4948. This article references the use of Phospho-eIF4E (Ser209) Antibody in the following applications: Western Blotting
- Lindemann, S. et al. (2001) Integrins regulate the intracellular distribution of eukaryotic initiation factor 4E in platelets: A checkpoint for translational control. J. Biol. Chem. 276, 33947-33951. This article references the use of Phospho-eIF4E (Ser209) Antibody in the following applications: Western Blotting
- Pyronnet, S. et al. (2001) Suppression of cap-dependent translation in mitosis. Genes Dev. 15, 2083-2093. This article references the use of Phospho-eIF4E (Ser209) Antibody in the following applications: Western Blotting
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Companion Products
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- 4923 Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
- 2441 Phospho-eIF4G (Ser1108) Antibody
- 2498 eIF4G Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
