Product Pathways - Translational Control

eIF4E Antibody #9742

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P IHC-F	H M R Mk Z	Endogenous	25	Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Z=Zebra Fish
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

eIF4E Antibody detects endogenous levels of total eIF4E protein. The antibody does not cross-react with other proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding Ser209 of eIF4E. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
5. Raught, B. et al. (2000) EMBO J. 19, 434-444.

Application References

• Krichevsky, A.M. and Kosik, K.S. (2001) Neuronal RNA granules: A link between RNA localization and stimulation-dependent translation. Neuron 32, 683-696. This article references the use of eIF4E Antibody in the following applications: Western Blotting
• Banerjee, S. et al. (2002) Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937-5948. This article references the use of eIF4E Antibody in the following applications: Western Blotting

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Companion Products

• 2067 eIF4E (C46H6) Rabbit mAb
• 9741 Phospho-eIF4E (Ser209) Antibody
• 2441 Phospho-eIF4G (Ser1108) Antibody
• 2498 eIF4G Antibody
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• 9455 Phospho-4E-BP1 (Thr70) Antibody
• 2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
• 4923 Nonphospho-4E-BP1 (Thr46) (87D12) Rabbit mAb
• 9644 4E-BP1 (53H11) Rabbit mAb
• 2111 Phospho-Mnk1 (Thr197/202) Antibody
• 4992 PABP1 Antibody
• 4763 Translation Initiation Complex Antibody Sampler Kit
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.