Product Pathways - Apoptosis / Autophagy
Caspase-8 (1C12) Mouse mAb #9746
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H | 18, 43, 57 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Caspase-8 (1C12) Mouse mAb detects endogenous levels of full length caspase-8 (57 kDa), the cleaved intermediate p43/p41 and the caspase-8 active fragment p18. This antibody does not cross-react with other caspases.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) derived from the carboxy-terminal sequence of the p18 fragment of human caspase-8. Antibody is supplied in HEPES buffer with 50% glycerol and less than 0.02% sodium azide.
Western Blotting
Western blot analysis of extracts from SKW6.4 cells, untreated or anti-Fas-treated (1 µg/ml), and Jurkat cells, untreated or etoposide-treated (25 µM), using Caspase-8 (1C12) Mouse mAb.
Background
Apoptosis induced through CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6 and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
- Muzio, M. et al. (1996) Cell 85, 817-827.
- Boldin, M.P. et al. (1996) Cell 85, 803-815.
- Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.
Application References
- Kumar-Sinha, C. et al. (2002) Molecular cross-talk between the TRAIL and interferon signaling pathways. J. Biol. Chem. 277 (1), 575-585. This article references the use of Caspase-8 (1C12) Mouse mAb in the following applications: Western Blotting
- Yu, W. et al. (2003) RRR-alpha-tocopheryl succinate-induced apoptosis of human breast cancer cell involves Bax translocation to mitochondria. Cancer Res. 63 (10), 2483-91. This article references the use of Caspase-8 (1C12) Mouse mAb in the following applications: Western Blotting
- Jeong, W. et al. (2004) Roles of TRP14, a thioredoxin-related protein in tumor necrosis factor-alpha signaling pathways. J. Biol. Chem. 279, 3151-3159. This article references the use of Caspase-8 (1C12) Mouse mAb in the following applications: Western Blotting
- Mongini, P. K. et al. (2004) Role of complement-binding CD21/CD19/CD81 in enhancing human B cell protection from Fas-mediated apoptosis. J. Immunol. 171, 5244-5254. This article references the use of Caspase-8 (1C12) Mouse mAb in the following applications: Western Blotting
- Chandrasekar, B. et al. (2004) Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death. J Biol Chem. 279 (19), 20221-33. This article references the use of Caspase-8 (1C12) Mouse mAb in the following applications: Western Blotting
- Ballestrero, A. et al. (2004) Tumor necrosis factor-related apoptosis-inducing ligand cooperates with anticancer drugs to overcome chemoresistance in antiapoptotic Bcl-2 family members expressing jurkat cells. Clin. Cancer Res. 10, 1463-1470. This article references the use of Caspase-8 (1C12) Mouse mAb in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
