Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb #9748

Applications Reactivity MW (kDa) Source Isotype
W H 10 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb detects endogenous levels of the small fragment of caspase-8 resulting from cleavage at aspartic acid 384. The antibody does not cross-react with full length caspase-8.

Source / Purification

Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) derived from the amino-terminal sequence of p10 of human caspase-8. Antibody is supplied in HEPES buffer with 50% glycerol and less than 0.02% sodium azide.

Western Blotting

Western Blotting

Western blot analysis of extracts from SKW6.4 cells, untreated or anti-Fas-treated (1 µg/ml), using Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb (left) or Caspase-8 (1C12) Mouse mAb #9746 (right).

Background

Apoptosis induced through CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6 and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

  1. Muzio, M. et al. (1996) Cell 85, 817-827.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-815.
  3. Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.

Application References

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