Product Pathways - Apoptosis
Cleaved Caspase-6 (Asp162) Antibody #9761
|9761S||100 µl (10 western blots)||---||In Stock||---|
|9761P||40 µl (4 western blots)||---||In Stock||---|
|9761||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||18||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Cleaved Caspase-6 (Asp162) Antibody detects endogenous levels of the large subunit of active caspase-6 resulting from cleavage at Asp179 in human or Asp162 in mouse and rat (18 kDa). This antibody does not recognize other cleaved caspases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of p18, the large subunit of rat caspase-6. Antibodies are purified by protein A and peptide affinity chromatography.
Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.
- Bhakar, A. L. et al. (2003) J Neurosci. 23 (36), 11373-81. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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