Product Pathways - Apoptosis / Autophagy
Caspase-6 Antibody #9762
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R | 15, 35 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35 kDa) and the small subunit of caspase-6 resulting from cleavage at aspartic acid 194 (15 kDa). This antibody does not recognize other caspases.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding the cleavage site of caspase-6. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or staurosporine-treated (1 µM) and Jurkat cells, untreated or etoposide-treated (25 µM), using Caspase-6 Antibody.
Background
Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.
- Cohen, G.M. (1997) Biochem. J. 326, 1-16.
- Faleiro, L. et al. (1997) EMBO J. 16, 2271-2281.
- Li, P. et al. (1997) Cell 91, 479-489.
- Slee, E. A. et al. (1999) J. Cell. Biol. 144, 281-292.
- MacFarlane, M. et al. (1997) J. Cell. Biol. 137, 469-479.
- Orth, K. et al. (1996) J. Biol. Chem. 271, 16443-16446.
Application References
- Hara, H. et al. (2002) The apoptotic protease-activating factor 1-mediated pathway of apoptosis is dispensable for negative selection of thymocytes. J. Immunol. 168, 2288-2295. This article references the use of Caspase-6 Antibody in the following applications: Western Blotting
- Schroder, A. et al. (2002) STAT6 serves as a positive and negative regulator of gene expression in IL-4-stimulated B lymphocytes. J. Immunol. 168, 996-1000. This article references the use of Caspase-6 Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
