Product Pathways - Tyrosine Kinase / Adaptors
14-3-3 Family Antibody Sampler Kit #9769
|9769S||1 Kit (6 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|14-3-3 β/α Antibody #9636||40 µl||W||H, M, R, Mk||28||Rabbit|
|14-3-3 γ (D15B7) Rabbit mAb #5522||40 µl||W||H, M, R, Mk, Pg||C, X, Z||27||Rabbit IgG|
|14-3-3 ζ/δ (D7H5) Rabbit mAb #7413||40 µl||W||H, M, R, Mk, Pg||C||28||Rabbit IgG|
|14-3-3 ε Antibody #9635||40 µl||W||H, M, R, Mk||28||Rabbit|
|14-3-3 τ Antibody #9638||40 µl||W, IHC-P||H, M, R, Mk||28||Rabbit|
|14-3-3 η (D23B7) Rabbit mAb #5521||40 µl||W||H, M, R, Mk, B, Pg||27||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Pg=Pig, B=Bovine
Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb #5521.
Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb #5522.
Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb #7413.
Western blot analysis of extracts from HeLa, NIH/3T3, PC12, and COS cells using 14-3-3 ε Antibody #9635.
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody #9636.
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody #9638.
The 14-3-3 Family Antibody Sampler Kit provides an economical means to investigate the expression of various 14-3-3 isoforms within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Specificity / Sensitivity
Each antibody in the 14-3-3 Family Antibody Sampler Kit detects endogenous levels of its respective target.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequences of human 14-3-3 ß/α, 14-3-3 ε and 14-3-3 τ proteins. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg80 of human 14-3-3 ζ/δ protein, Leu37 of human 14-3-3 η protein and Ile79 of human 14-3-3 γ protein.
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).
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