Product Pathways - Apoptosis
IAP Family Antibody Sampler Kit #9770
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|c-IAP1 (D5G9) Rabbit mAb #7065||40 µl||W IP||H (Mk)||62||Rabbit IgG|
|c-IAP2 (58C7) Rabbit mAb #3130||40 µl||W IP||H (Mk)||70||Rabbit IgG|
|Survivin (71G4B7) Rabbit mAb #2808||40 µl||W IP IHC-P IHC-F IF-IC F||H M R||16||Rabbit IgG|
|XIAP (3B6) Rabbit mAb #2045||40 µl||W||H Mk||53||Rabbit IgG|
|Livin (D61D1) XP® Rabbit mAb #5471||40 µl||W IP IF-IC||H||34, 36||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the IAP Family Antibody Sampler Kit detects endogenous levels of its respective target.
Western blot analysis of extracts from HeLa, Raji and COS cell lines, using XIAP (3B6) Rabbit mAb #2045.
Western blot analysis of lysates from Raji, BaF3, and C6 cell lines, using Survivin (71G4B7) Rabbit mAb #2808.
Western blot analysis of extracts from Raji, Ramos and SR cell lines, using c-IAP2 (58C7) Rabbit mAb #3130.
Western blot analysis of extracts from SK-MEL-28, Malme-3M, SK-MEL-2, and HeLa cells using Livin (D61D1) XP® Rabbit mAb #5471.
The IAP Family Antibody Sampler Kit provides an economical means to investigate the expression of various IAP family members within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein. The antibody is purified by protein A and affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val117 of human c-IAP2, Cys60 of human Survivin, Ser245 of human XIAP, and Ala195 of human Livin.
The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).
- Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
- Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
- Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
- Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
- Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
- Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
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For Research Use Only. Not For Use In Diagnostic Procedures.