Product Pathways - Apoptosis

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IAP Family Antibody Sampler Kit #9770

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
c-IAP1 (D5G9) Rabbit mAb #7065	40 µl	W IP	H (Mk)	62	Rabbit IgG
c-IAP2 (58C7) Rabbit mAb #3130	40 µl	W IP	H (Mk)	70	Rabbit IgG
Survivin (71G4B7) Rabbit mAb #2808	40 µl	W IP IHC-P IHC-F IF-IC F	H M R	16	Rabbit IgG
XIAP (3B6) Rabbit mAb #2045	40 µl	W	H Mk	53	Rabbit IgG
Livin (D61D1) XP® Rabbit mAb #5471	40 µl	W IP IF-IC	H	34, 36	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the IAP Family Antibody Sampler Kit detects endogenous levels of its respective target.

Description

The IAP Family Antibody Sampler Kit provides an economical means to investigate the expression of various IAP family members within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein. The antibody is purified by protein A and affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val117 of human c-IAP2, Cys60 of human Survivin, Ser245 of human XIAP, and Ala195 of human Livin.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).

1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.

Application References

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Protocols

2045:

Western Blotting

2808:

Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

3130:

Immunoprecipitation, Western Blotting

5471:

Immunofluorescence, Immunoprecipitation, Western Blotting

7065:

Immunoprecipitation, Western Blotting

Companion Products

• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.