Product Pathways - Antibody Sampler Kits
Loading Control Antibody Sampler Kit (Mouse) #9774
|9774S||1 Kit (3 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|β-Actin (8H10D10) Mouse mAb #3700||40 µl||W, IHC-P, IF-IC, F||H, M, R, Hm, Mk, Dg||45||Mouse IgG2b|
|α-Tubulin (DM1A) Mouse mAb #3873||40 µl||W, IHC-P, IF-IC||H, M, R, Mk||52||Mouse IgG1|
|Histone H3 (96C10) Mouse mAb #3638||40 µl||W||H, M, R, Mk||Dm, X, Hr||17||Mouse IgG1|
|Anti-mouse IgG, HRP-linked Antibody #7076||100 µl||Horse|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey, Dg=Dog
Western blot analysis of extracts from various cell lines using Histone H3 (96C10) Mouse mAb #3638.
Western blot analysis of extracts from various cell types using β-Actin (8H10D10) Mouse mAb #3700.
Western blot analysis of extracts from HeLa and COS-7 cells using α-Tubulin (DM1A) Mouse mAb #3873.
The Loading Control Antibody Sampler Kit provides an economical means to detect a variety of housekeeping proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.
Specificity / Sensitivity
Each antibody in the Loading Control Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross-react with other proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino terminus of human β-actin, the carboxy terminus of human histone H3, or with full-length chicken α-tubulin purfied from brain extracts. The epitope of α-Tubulin (DM1A) Mouse mAb recognizes residues surrounding Val440 of human α-tubulin protein.
Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, histone H3, and α-tubulin as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (2). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (3).
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* Product-specific protocol.
- 5142 Loading Control Antibody Sampler Kit
- 3700 β-Actin (8H10D10) Mouse mAb
- 3873 α-Tubulin (DM1A) Mouse mAb
- 3638 Histone H3 (96C10) Mouse mAb
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 9999 Nonfat Dry Milk
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
For Research Use Only. Not For Use In Diagnostic Procedures.
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