Cell Signaling Technology

Product Pathways - NF-kB Signaling

Pirin (1E8) Rat mAb #9777

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP F H M R Hm Mk B Endogenous 32 Rat IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Pirin (1E8) Rat mAb detects endogenous levels of Pirin protein. This antibody does not cross-react with other proteins in the cupin superfamily.

Source / Purification

Monoclonal antibody is produced by immunizing animals with the full-length Pirin protein.

Western Blotting

Western Blotting

Western blot analysis of cell lysates from HeLa, NIH/3T3, C6 and COS cells, using Pirin (1E8) Rat mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using Pirin (1E8) Rat mAb (blue) compared to a nonspecific negative control antibody (red).

Background

Pirin is a highly conserved nuclear protein and a member of the cupin superfamily of proteins, all of which contain two conserved β-barrel fold domains (1). Pirin functions as a co-factor for NFI/CTF1 and Bcl-3, implicating it in DNA replication, transcriptional activation and apoptosis (2,3). Both human and bacterial pirins catalyze the di-oxygenation of quercetin, one of a class of widespread naturally occurring flavenoid compounds that have anti-inflammatory and anti-cancer activities (4). Flavenoids exert these beneficial activities by functioning as antioxidants that stabilize cellular free radical molecules and by directly modulating cell signaling pathways involving PI 3-kinase, Akt/PKB, PKC and MAP kinases (5). Quercetin has also been directly implicated in the regulation of NF-κB activity; thus, Pirin may exert its apoptotic functions both by directly regulating Bcl-3/NF-κB activity and by modulating quercetin levels in the cell (6).

  1. Dunwell, J.M. et al. (2001) Trends Biochem. Sci. 26, 740-746.
  2. Wendler, W.M. et al. (1997) J. Biol. Chem. 272, 8482-8489.
  3. Dechend, R. et al. (1999) Oncogene 18, 3316-3323.
  4. Adams, M. and Jia, Z. (2005) J. Biol. Chem. 280, 28675-28682.
  5. Williams, R.J. et al. (2004) Free Radic. Biol .Med. 36, 838-849.
  6. Chen, C.C. et al. (2004) Mol. Pharmacol. 66, 683-693.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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