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Rag Protein Antibody Sampler Kit #9778

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
Raptor (24C12) Rabbit mAb #2280	40 µl	W IP	H M R Mk	150	Rabbit IgG
mTOR (7C10) Rabbit mAb #2983	40 µl	W IHC-P IF-IC F	H M R Mk (Hr)	289	Rabbit IgG
RagB Antibody #3830	40 µl	W	H	40	Rabbit
RagA (D8B5) Rabbit mAb #4357	40 µl	W IP	H M R Mk	30	Rabbit IgG
RagD Antibody #4470	40 µl	W	H	50	Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat
RagC (D31G9) XP® Rabbit mAb #5466	40 µl	W IP IHC-P	H M R Mk	50	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hr=Horse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Rag Protein Antibody Sampler Kit detects endogenous levels of its target protein. RagA (D8B5) Rabbit mAb may also detect endogenous levels of total RagB protein.

Description

The Rag Protein Antibody Sampler Kit provides an economical means to evaluate the regulation of mTORC1 signaling by such proteins as Raptor, RagA, RagB, RagC, and RagD. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues in the human sequences for RagB, and RagD proteins, andcorresponding to residues near the amino terminus of human RagC protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues of human mTOR, RagA, and Raptor.

Background

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

2280:

Immunoprecipitation, Western Blotting

2983:

Flow, IHC / Paraffin, Immunofluorescence, Western Blotting

3830:

Western Blotting

4357:

Immunoprecipitation, Western Blotting

4470:

Western Blotting

5466:

IHC / Paraffin, Immunoprecipitation, Western Blotting

Companion Products

• 2280 Raptor (24C12) Rabbit mAb
• 2983 mTOR (7C10) Rabbit mAb
• 3360 RagC Antibody
• 3830 RagB Antibody
• 4357 RagA (D8B5) Rabbit mAb
• 4470 RagD Antibody
• 2971 Phospho-mTOR (Ser2448) Antibody
• 9862 mTOR Substrates Antibody Sampler Kit
• 9864 mTOR Regulation Antibody Sampler Kit
• 9964 mTOR Pathway Antibody Sampler Kit
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7075 Anti-biotin, HRP-linked Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7727 Biotinylated Protein Ladder Detection Pack

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For Research Use Only. Not For Use In Diagnostic Procedures.