Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

PP2A Antibody Sampler Kit #9780

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
PP2A A Subunit (81G5) Rabbit mAb #2041 40 µl W IHC-P IF-IC H M R Mk 62 Rabbit IgG
PP2A B Subunit (100C1) Rabbit mAb #2290 40 µl W IP IHC-P H M R Mk Dm (C) (B) (Pg) 52 Rabbit IgG
PP2A C Subunit (52F8) Rabbit mAb #2259 40 µl W IP IHC-P F H M R Mk Dm (C) (Pg) 36, 38 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  Dm=D. melanogaster  B=Bovine  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

PP2A A Subunit (81G5) Rabbit mAb detects endogenous levels of α and β isoforms of the PP2A A subunit and does not cross-react with other PP2A subunits. PP2A B Subunit (100C1) Rabbit mAb detects endogenous levels of the α isoform of PP2A B and may recognize the β, γ, and δ isoforms of PP2A B. The antibody does not cross-react with the B', B'' or B''' families of PP2A B subunits. PP2A C Subunit (52F8) Rabbit mAb detects endogenous levels of α and β isoforms of the PP2A catalytic subunit and does not cross-react with other PP2A subunits.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PP2A A Subunit (81G5) Rabbit mAb #2041.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PP2A C Subunit (52F8) Rabbit mAb #2259.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PP2A B Subunit (100C1) Rabbit mAb #2290.


Description

The PP2A Antibody Sampler Kit provides an economical means of evaluating PP2A protein. The kit contains enough primary and secondary antibodies to perform four western blots with each antibody.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human PP2A A, B, or C subunit proteins.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).

  1. Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
  2. Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
  3. Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
  4. Chen, J. et al. (1992) Science 257, 1261-1264.
  5. Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
  6. Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
  7. Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
  8. Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products

Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products