Product Pathways - Cell Cycle / Checkpoint
PP2A Antibody Sampler Kit #9780
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|PP2A A Subunit (81G5) Rabbit mAb #2041||40 µl||W IHC-P IF-IC||H M R Mk||62||Rabbit IgG|
|PP2A B Subunit (100C1) Rabbit mAb #2290||40 µl||W IP IHC-P||H M R Mk Dm (C) (B) (Pg)||52||Rabbit IgG|
|PP2A C Subunit (52F8) Rabbit mAb #2259||40 µl||W IP IHC-P F||H M R Mk Dm (C) (Pg)||36, 38||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Dm=D. melanogaster B=Bovine Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
PP2A A Subunit (81G5) Rabbit mAb detects endogenous levels of α and β isoforms of the PP2A A subunit and does not cross-react with other PP2A subunits. PP2A B Subunit (100C1) Rabbit mAb detects endogenous levels of the α isoform of PP2A B and may recognize the β, γ, and δ isoforms of PP2A B. The antibody does not cross-react with the B', B'' or B''' families of PP2A B subunits. PP2A C Subunit (52F8) Rabbit mAb detects endogenous levels of α and β isoforms of the PP2A catalytic subunit and does not cross-react with other PP2A subunits.
Western blot analysis of extracts from various cell lines using PP2A A Subunit (81G5) Rabbit mAb #2041.
Western blot analysis of extracts from various cell lines using PP2A C Subunit (52F8) Rabbit mAb #2259.
Western blot analysis of extracts from various cell lines using PP2A B Subunit (100C1) Rabbit mAb #2290.
The PP2A Antibody Sampler Kit provides an economical means of evaluating PP2A protein. The kit contains enough primary and secondary antibodies to perform four western blots with each antibody.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human PP2A A, B, or C subunit proteins.
Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
- Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
- Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
- Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
- Chen, J. et al. (1992) Science 257, 1261-1264.
- Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
- Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
- Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
- Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.
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- 2260 PP2A A Subunit (6G3) Rat mAb
- 2039 PP2A A Subunit Antibody
- 1054 PP2A A Subunit Blocking Peptide
- 4953 PP2A B Subunit Antibody
- 1051 PP2A B Subunit Blocking Peptide
- 2038 PP2A C Subunit Antibody
- 1067 PP2A C Subunit Blocking Peptide
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.