Product Pathways - Cell Cycle / Checkpoint
Mismatch Repair Antibody Sampler Kit #9786
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| MLH1 (4C9C7) Mouse mAb #3515 | 40 µl | W IP IF-IC F | H Mk | 85 | Mouse IgG1 |
| MSH2 (D24B5) XP® Rabbit mAb #2017 | 40 µl | W IP IHC-P IF-IC F | H M | 100 | Rabbit IgG |
| MSH6 (L990) Antibody #3996 | 40 µl | W IF-IC | H Mk | 160 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl | Horse |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein. MSH2 (D24B5) XP® Rabbit mAb detects endogenous levels of total MSH2 protein. MSH6 (L990) Antibody detects endogenous levels of total MSH6 protein.
Western Blotting
Western blot analysis of extracts from HeLa and NIH/3T3 cells using MSH2 (D24B5) XP® Rabbit mAb #2017.
Western Blotting
Western blot analysis of extracts from various cell lines using MLH1 (4C9C7) Mouse mAb #3515.
Western Blotting
Western blot analysis of extracts from various cell lines using MSH6 (L990) Antibody #3996.
Description
Mismatch Repair Antibody Sampler Kit provides an economical means to investigate the DNA mismatch repair system within the cell. The kit contains primary and secondary antibodies to perform four western blots with each antibody.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with truncated recombinant MBP-MLH1 or by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human MSH2. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLH6 protein. Antibodies are purified using protein A and peptide affinity chromatography.
Background
The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).
- O'Brien, V. and Brown, R. (2006) Carcinogenesis 27, 682-92.
- Wang, Y. et al. (2000) Genes Dev 14, 927-39.
- Plotz, G. et al. (2006) J Mol Histol 37, 271-83.
- Yip, S. et al. (2009) Clin Cancer Res 15, 4622-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 2017:
- Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 3515:
- Flow, Immunofluorescence, Immunoprecipitation, Western Blotting
- 3996:
- Immunofluorescence, Western Blotting
Companion Products
- 9947 DNA Damage Antibody Sampler Kit
- 9919 Phospho-p53 Antibody Sampler Kit
- 9917 Cell Cycle/Checkpoint Antibody Sampler Kit
- 8342 UV Induced DNA Damage Response Antibody Sampler Kit
- 8344 MRN Complex Antibody Sampler Kit
- 9931 Phospho-Chk1/2 Antibody Sampler Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
