Product Pathways - Chromatin Regulation / Epigenetics
Polycomb Group Antibody Sampler Kit #9788
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| SUZ12 (D39F6) XP® Rabbit mAb #3737 | 40 µl | W IP IF-IC ChIP | H M R Mk (Pg) (Hr) | 83 | Rabbit IgG |
| Ezh2 (D2C9) XP® Rabbit mAb #5246 | 40 µl | W IP IHC-P IF-IC ChIP | H M R Mk | 98 | Rabbit IgG |
| Ring1A Antibody #2820 | 40 µl | W | H M R Mk | 58 | Rabbit |
| RING1B (D22F2) XP® Rabbit mAb #5694 | 40 µl | W IP IF-IC ChIP | H M R Mk | 41 | Rabbit IgG |
| Bmi1 (D20B7) XP® Rabbit mAb #6964 | 40 µl | W IP IHC-P IF-IC ChIP | H Mk | 41, 43 | Rabbit IgG |
| Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 | 40 µl | W IP IHC-P IF-IC ChIP | H M R Mk (X) (Z) | 17 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
ChIP=Chromatin IP
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
X=Xenopus
Z=Zebrafish
Pg=Pig
Hr=Horse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the Polycomb Group Antibody Sampler Kit detects endogenous levels of target proteins.
Western Blotting
Western blot analysis of extracts of MOLT4 and LNCaP cells using Ring1A Antibody #2820.
Western Blotting
Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP® Rabbit mAb #3737.
Western Blotting
Western blot analysis of extracts from MCF7, Neuro-2a, and COS-7 cell lines using Ezh2 (D2C9) XP® Rabbit mAb #5246.
Western Blotting
Western blot analysis of extracts from various cell lines using RING1B (D22F2) XP® Rabbit mAb #5694.
Western Blotting
Western blot analysis of extracts from various cell lines using Bmi1 (D20B7) XP® Rabbit mAb #6964.
Western Blotting
Western blot analysis of various cell lines using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733.
Description
The Polycomb Group Antibody Sampler Kit provides an economical means of evaluating total levels of Polycomb Group Proteins. The kit contains enough primary and secondary antibodies to perform four western mini-blot experiments.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Arg354 of human Ezh2, the human SUZ12 protein, the carboxy terminus of human Bmi1 protein, the amino terminus of histone H3 in which Lys27 is tri-methylated, or with recombinant protein specific to the full-length human RING1B protein, respectively. Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to human RING1A. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.
Background
The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell-cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, Eed-Ezh2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinates histone H2A on Lys119 (6). PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of an additional catalytic subunit RING1B (also RING2 or RNF2) (7). PcG proteins play an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and are required for maintenance of adult hematopoietic and neural stem cells, as well as embryonic stem cells (3,4,8-10).
- Boyer, L.A. et al. (2006) Nature 441, 349-53.
- Lee, T.I. et al. (2006) Cell 125, 301-13.
- Park, I.K. et al. (2003) Nature 423, 302-5.
- Molofsky, A.V. et al. (2003) Nature 425, 962-7.
- Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
- Wang, H. et al. (2004) Nature 431, 873-8.
- Cao, R. et al. (2005) Mol Cell 20, 845-54.
- Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
- Jacobs, J.J. et al. (1999) Nature 397, 164-8.
- Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 2820:
- Western Blotting
- 3737:
- ChIP Agarose, ChIP Magnetic, Immunofluorescence, Immunoprecipitation, Western Blotting
- 5246:
- ChIP Agarose, ChIP Magnetic, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 5694:
- ChIP Agarose, ChIP Magnetic, Immunofluorescence, Immunoprecipitation, Western Blotting
- 6964:
- ChIP Agarose, ChIP Magnetic, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 9733:
- ChIP Agarose, ChIP Magnetic, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
Companion Products
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
