Product Pathways - Phosphatases
SHP-2 Antibody Sampler Kit #9793
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-SHP-2 (Tyr542) Antibody #3751||40 µl||W IP||H M R (Mk) (C) (X)||72||Rabbit|
|SHP-2 (D50F2) Rabbit mAb #3397||40 µl||W IP||H M R Mk||72||Rabbit IgG|
|Phospho-SHP-2 (Tyr580) Antibody #3703||40 µl||W IP||H M R (C)||72||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken X=Xenopus
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
SHP-2 (D50F2) Rabbit mAb detects endogenous levels of total SHP-2 protein. Phospho- (Tyr542) SHP-2 Antibody detects endogenous SHP-2 only when phosphorylated at the specified at Tyr542. Phospho- SHP-2 (Tyr580) Antibody detects endogenous level of SHP2 only when phosphorylated at Tyr580. Some cross-reactivity with phosphorylated RTKs may occur. See individual product webpages for more information.
Western blot analysis of extracts from various cell lines using SHP-2 (D50F2) Rabbit mAb #3397.
Western blot analysis of total cell lysates from NIH/3T3 cells, serum-starved overnight and treated with PDGF (#9909) for 5 minutes, using Phospho-SHP-2 (Tyr580) Antibody #3703 (upper) or control SHP-2 Antibody #3752 (lower).
Western blot analysis of extracts from C6 and NIH/3T3 cells, starved for 18 hours and either untreated or PDGF-treated (50ng/ml, 20 minutes), using Phospho-SHP-2 (Tyr542) Antibody #3751 (upper) or total SHP-2 Antibody #3752 (lower).
The SHP-2 Antibody Sampler Kit provides an economical means to evaluate levels of SHP-2 protein phosphorylated at the specified sites, as well as total SHP-2 levels. The kit contains enough primary and secondary antibody to perform four western blot experiments per antibody.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human SHP-2. Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr542 and Tyr580 of human SHP-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 at p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6).
- Qu, C.K. (2000) Cell Res 10, 279-88.
- Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25.
- Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72.
- Bennett, A.M. et al. (1994) Proc Natl Acad Sci USA 91, 7335-9.
- Lu, W. et al. (2001) Mol Cell 8, 759-69.
- Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90.
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- 3752 SHP-2 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 7722 Blue Loading Buffer Pack
- 7723 Red Loading Buffer Pack
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.