Product Pathways - Tyrosine Kinase / Adaptors
c-Cbl Antibody Sampler Kit #9794
|9794S||1 Kit (3 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|c-Cbl (C49H8) Rabbit mAb #2179||40 µl||W, IP||H, Mk||120||Rabbit IgG|
|Phospho-c-Cbl (Tyr731) Antibody #3554||40 µl||W||H||120||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb #8869||40 µl||W, IP||H||M||120||Rabbit IgG|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, Mk=Monkey
Western blot analysis of cell extracts from various cell types using c-Cbl (C49H8) Rabbit mAb #2179.
Western blot analysis of extracts from 293-T cells transfected with c-Cbl and Fyn (lanes 3 and 6) or without Fyn (lanes 2 and 5), untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-c-Cbl (Tyr731) Antibody #3554. (Lysates provided by Dr. A.L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.)
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with pervanadate (1 mM; +), using Phospho-c-Cbl (Tyr700) (D16D7) Rabbit mAb #8869 (upper) or c-Cbl (D4E10) Rabbit mAb #8447 (lower).
The c-Cbl Antibody Sampler Kit provides an economical means of evaluating levels of c-Cbl protein phosphorylated at the specified sites, as well as total c-Cbl levels. The kit contains enough reagents to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
The Phospho-c-Cbl (Tyr700) and (Tyr731) Antibodies detect endogenous c-Cbl when phosphorylated at the specified sites. c-Cbl (C49H3) Rabbit mAb detects endogenous levels of total c-Cbl protein.
Source / Purification
The phospho-specific polyclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr731 of human c-Cbl. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. The monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human c-Cbl and residues surrounding Tyr700 of human c-Cbl.
The c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells (1,2). c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol-3 kinase, Crk, and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. The RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs (3). Research studies have indicated that in human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5). Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn (6).
- Blake, T.J. et al. (1991) Oncogene 6, 653-657.
- Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.
- Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294-307.
- Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323-8331.
- Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.
- Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.