Cell Signaling Technology

Product Pathways - Metabolism

ACAD9 Antibody #9796

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 69 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ACAD9 Antibody recognizes endogenous levels of total ACAD9 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val512 of human ACAD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ACAD9 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human ACAD9 (hACAD9-DDK; +), using ACAD9 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

The mitochondrial flavoenzymes acyl-CoA dehydrogenases (ACADs) catalyze the α,β dehydrogenation of acyl-CoA esters (1). One of these enzymes, ACAD9, is essential for assembly of oxidative phosphorylation complex I (2). Studies have shown that ACAD9 gene mutations cause Complex I deficiency (2,3).

  1. He, M. et al. (2007) Am J Hum Genet 81, 87-103.
  2. Nouws, J. et al. (2010) Cell Metab 12, 283-94.
  3. Haack, T.B. et al. (2010) Nat Genet 42, 1131-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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