Kinase Buffer (10X) #9802
Kinase Buffer can be used to assay protein kinase activity.
Directions for Use
1. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at –20°C. Aliquotting of 10X buffer is recommended if many small experiments are to be performed.
2. Thaw 10X buffer at 24-30°C, mixing end-over-end.
3. Dilute the 10X buffer to the desired concentration using ddH2O.
Solutions and Reagents
1X Kinase Buffer
25 mM Tris-HCl (pH 7.5), 5 mM beta-glycerophosphate, 2 mM dithiothreitol (DTT), 0.1 mM Na3VO4, 10 mM MgCl2.
Note: Kinase Buffer must be supplemented with ATP (#9804).
- Bisikirska, B.C. et al. (2012) Oncogene , . Applications: Kinase Assay
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For Research Use Only. Not For Use In Diagnostic Procedures.