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Kinase Buffer (10X) #9802

Description

Kinase Buffer can be used to assay protein kinase activity.

Directions for Use

1. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at –20°C. Aliquotting of 10X buffer is recommended if many small experiments are to be performed.

2. Thaw 10X buffer at 24-30°C, mixing end-over-end.

3. Dilute the 10X buffer to the desired concentration using ddH2O.

Solutions and Reagents

1X Kinase Buffer

25 mM Tris-HCl (pH 7.5), 5 mM beta-glycerophosphate, 2 mM dithiothreitol (DTT), 0.1 mM Na3VO4, 10 mM MgCl2.

Note: Kinase Buffer must be supplemented with ATP (#9804).

Application References

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Companion Products

• 9803 Cell Lysis Buffer (10X)
• 9804 ATP (10 mM)

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.