Product Pathways - MAPK Signaling
c-Jun Fusion Protein Beads #9811
Molecular Weight
35 kDa
Description
c-Jun Fusion Protein Beads selectively "pull down" SAPK/JNK and also serve as a useful substrate to measure SAPK/JNK activity. It is expressed as a recombinant protein fusion of c-Jun codons 1?89 and glutathione S-transferase. The fusion protein is produced in E. coli and purified by glutathione sepharose beads.It is supplied as a 50% slurry* of GST-c-Jun beads.
Directions for Use
Prior to use, put the tube on ice for 5 minutes to lower viscosity of buffer. Then beads should be resuspended to a 50% slurry by inversion or gentle vortexing.
Background
c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).
- Binetruy, B. et al. (1991) Nature 351, 122-127.
- Smeal, T. et al. (1991) Nature 354, 494-496.
- Derijard, B. et al. (1994) Cell 76, 1025-1037.
- Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
Application References
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Companion Products
- 9810 SAPK/JNK Kinase Assay Kit (Nonradioactive)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
