Product Pathways - Screening Technologies
KinomeView® Profiling Kit #9812
For more information about this product, please email us at ptmscan@cellsignal.com.
† Select antibodies contained in the KinomeView® Profiling Kit are not available for individual sale.
Protocol
- 9812:
- KinomeView® Protocol
Specificity / Sensitivity
Each antibody recognizes endogenous levels of the respective target motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Western Blotting
Western blot analysis of extracts from various cell types, untreated or treated as indicated, using KinomeView™ Profiling Kit component antibodies. Select abbreviations: TBB, CKII inhibitor 4,5,6,7-tetrabromobenzotriazole; PDBu, phorbol 12,13-dibutyrate.
Phospho-MAPK/CDK Substrate antibody has broad reactivity with proteins phosphorylated on Ser residues with proximal Pro residues at the -2 and +1 positions. CMGC family kinases including MAPKs and CDKs phosphorylate substrates containing this motif. Changes in phosphorylation of this motif are detected with nocodazole mediated mitotic arrest, as well as MEK/ERK signaling inhibition by U0126 in HeLa cells.
Phospho-Tyr antibody reacts broadly with Tyr-phosphorylated proteins. HeLa cells were treated by different conditions, an induction in Tyr phosphorylation is observed upon stimulation of EGF receptor by treatment with EGF; EGFR mediated phosphorylation is inhibited by the tyrosine kinase inhibitor gefitinib.
Description
The KinomeView® Profiling Kit provides a set of Phospho-Motif Antibodies that cover a large portion of the kinome and react broadly with serine, threonine, and tyrosine phosphorylation mediated by diverse kinase families throughout the kinome. This kit will provide researchers with an immunoblotting strategy to dissect the complexity of the phosphoproteome and determine the kinase families involved in the regulation of diverse physiological processes. By using the provided Phospho-motif Antibodies, the investigator can assess global changes in protein phosphorylation by western analysis across a range of experimental samples and conditions.
Background
Cell Signaling Technology (CST) has developed Phospho-motif Antibodies that have broad immunoreactivity for serine, threonine and tyrosine phosphorylation mediated by both known and unknown kinases. Using these Motif Antibodies for western blotting provides a Kinome-wide view of cellular phosphorylation.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 5301 PathScan® Multiplex Western Cocktail I: Phospho-p90RSK, Phospho-Akt, Phospho-p44/42 MAPK (Erk1/2) and Phospho-S6 Ribosomal Protein Detection Cocktail I
- 5564 PTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit
- 5561 PTMScan® Phospho-Akt Substrate Motif mAb 1 (RXXS*/T*) Kit
- 5563 PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit
- 4652 PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) Kit
- 5565 PTMScan® Phospho-PKA Substrate Motif (RRXS*/T*) Kit
- 5566 PTMScan® Phospho-ST*P Motif (ST*P) XP® Kit
- 5636 PTMScan® Phospho-Tyrosine Mouse mAb (P-Tyr-100) Kit
- 5562 PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit
Selected rabbit monoclonal antibodies are produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
