Product Pathways - Metabolism
Arginase-1 Antibody #9819
|W||H M R||Endogenous||40||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Arginase-1 Antibody recognizes endogenous levels of total arginase-1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from mouse liver, rat liver, and Huh7 cells using Arginase-1 Antibody.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human arginase-1 (hArginase-1-DDK, +), using Arginase-1 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine (1). Recent studies show that intracellular pathogens induce the expression of arginase-1 in mouse macrophages through the Toll-like receptor (TLR) pathway; deletion of this enzyme in macrophages favors host survival (2). In a separate study, the expression of arginase-1 in macrophages was shown to inhibit Th2-dependent inflammation and fibrosis (3).
- Wu, G. and Morris, S.M. (1998) Biochem J 336 ( Pt 1), 1-17.
- El Kasmi, K.C. et al. (2008) Nat Immunol 9, 1399-406.
- Pesce, J.T. et al. (2009) PLoS Pathog 5, e1000371.
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For Research Use Only. Not For Use In Diagnostic Procedures.