Cell Signaling Technology

Product Pathways - Metabolism

Arginase-1 Antibody #9819

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Arginase-1 Antibody recognizes endogenous levels of total arginase-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse liver, rat liver, and Huh7 cells using Arginase-1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human arginase-1 (hArginase-1-DDK, +), using Arginase-1 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine (1). Recent studies show that intracellular pathogens induce the expression of arginase-1 in mouse macrophages through the Toll-like receptor (TLR) pathway; deletion of this enzyme in macrophages favors host survival (2). In a separate study, the expression of arginase-1 in macrophages was shown to inhibit Th2-dependent inflammation and fibrosis (3).

  1. Wu, G. and Morris, S.M. (1998) Biochem J 336 ( Pt 1), 1-17.
  2. El Kasmi, K.C. et al. (2008) Nat Immunol 9, 1399-406.
  3. Pesce, J.T. et al. (2009) PLoS Pathog 5, e1000371.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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