Product Pathways - PI3K / Akt Signaling
GSK-3β (3D10) Mouse mAb #9832
|9832S||100 µl (10 western blots)||---||In Stock||---|
|9832||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||46||Mouse IgG2a|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
GSK-3β (3D10) Mouse mAb recognizes endogenous levels of total GSK-3β protein. This antibody does not cross-react with GSK-3α.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy-terminus of human GSK-3β protein.
Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) and HeLa cells using GSK-3β (3D10) Mouse mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Flow cytometric analysis of wild-type mouse embryonic fibroblasts (MEFs) (green) and GSK-3β (-/-) MEFs (blue) using GSK-3β (3D10) Mouse mAb. (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Confocal immunofluorescent analysis of wild-type mouse embryonic fibroblasts (MEFs) (left) and GSK-3β (-/-) MEFs (right) using GSK-3β (3D10) Mouse mAb (green). Red = Propidium Iodide/RNase (fluorescent DNA dye). (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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