Product Pathways - Protein Folding/Stability
Geldanamycin #9843
Directions for Use
Geldanamycin is supplied as 600 μg powder. Store at or below -20°C. Before usage, dissolve the chemical in 1.08 ml DMSO to make 1 mM Geldanamycin. For working concentrations of 300 nM-1 μM, dilute 1:3000 to 1:1000. Treat cells with the desired concentration for 20-48 hours. Store liquid form at or below -20°C.
Note: Geldanamycin is not stable in liquid form (quality decreased in 1-2 weeks). Use freshly dissolved geldanamycin each time.
Western Blotting
Western blot analysis of extracts from HEK293 cells, untreated or treated with indicated concentrations of Geldanamycin for 20 hours, using IKKα Antibody #2682 showing IKKα degradation (upper). β-Actin Antibody #4967 was used as a loading control (lower).
Sequence
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2345 Chk1 Antibody
- 2682 IKKα Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9803 Cell Lysis Buffer (10X)
- 7722 Blue Loading Buffer Pack
- 4967 β-Actin Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
