Product Pathways - Protein Folding
Geldanamycin #9843
Directions for Use
Geldanamycin is supplied as 600 μg powder. Store at or below -20ºC. Before usage, dissolve the chemical in 1.08 ml DMSO to make 1 mM Geldanamycin. For working concentrations of 300 nM-1 μM, dilute 1:3000 to 1:1000. Treat cells with the desired concentration for 20-48 hours. Store liquid form at or below -20ºC.
Note: Geldanamycin is not stable in liquid form (quality decreased in 1-2 weeks). Use freshly dissolved geldanamycin each time.
Western Blotting
Western blot analysis of extracts from HEK293 cells, untreated or treated with indicated concentrations of Geldanamycin for 20 hours, using IKKα Antibody #2682 showing IKKα degradation (upper). β-Actin Antibody #4967 was used as a loading control (lower).
Structure
Structure of Geldanamycin
Background
Geldanamycin (GA) is a naturally existing HSP90 inhibitor that belongs to the benzoquinone ansamycin family. GA binds to the amino terminal ATP-binding pocket of HSP90 and inhibits ATP binding and hydrolysis. HSP90 is a chaperone interacting with a wide variety of important target proteins for cell signaling and regulation during tumorgenesis (1,2). The binding of GA to HSP90 interferes with HSP-mediated target protein folding, leading to target aggregation and degradation (1-3). GA and its synthetic derivatives show higher affinity to HSP90 in tumor cells as compared to normal tissues and constitute a class of protential antitumor drugs (2-3).
- Ochel, H.J. et al. (2001) Cell Stress Chaperones 6, 105-112.
- Sharp, S. and Workman, P. (2006) Adv.Cancer Res. 95, 323-348.
- Kamal, A. et al. (2003) Nature 425, 407-410.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4967 β-Actin Antibody
- 2345 Chk1 Antibody
- 2682 IKKα Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9803 Cell Lysis Buffer (10X)
- 7722 Blue Loading Buffer Pack
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
