Product Pathways - Chromatin Regulation
Methyl-Histone H3 Antibody Sampler Kit #9847
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb # 9725 | 40 microliters | W IP IHC-P IF-IC ChIP | H M R Mk | 17 | Rabbit |
| Di-Methyl-Histone H3 (Lys9) Antibody # 9753 | 40 microliters | W IP IHC-P IF-IC ChIP | H M R Mk (All) | 17 | Rabbit |
| Di-Methyl-Histone H3 (Lys27) Antibody # 9755 | 40 microliters | W IP IF-IC | H M R Mk | 17 | Rabbit |
| Di-Methyl-Histone H3 (Lys36) Antibody # 9758 | 40 microliters | W IP IF-IC | H M R Mk (All) | 17 | Rabbit |
| Di-Methyl-Histone H3 (Lys79) Antibody # 9757 | 40 microliters | W IP | H M R Mk (All) | 17 | Rabbit |
| Histone H3 Antibody # 9715 | 40 microliters | W IP IHC-P IF-IC | H M R Mk B (Dr) | 17 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 100 microliters | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
ChIP=Chromatin IP
Reactivity Key:
H=Human
M=Mouse
R=Rat
All=All
Mk=Monkey
B=Bovine
Dr=Drosophila
Specificity / Sensitivity
All antibodies in the Methyl-Histone H3 Antibody Sampler Kit recognize histone H3 only when modified at the indicated site.
Western Blotting
Western blot analysis of extracts from various cell types using Histone H3 Antibody #9715.
Western Blotting
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725.
Western Blotting
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys9) Antibody #9753.
Western Blotting
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys27) Antibody #9755.
Western Blotting
Western blot analysis of extracts from various cell lines using Di-Methyl Histone H3 (Lys79) Antibody #9757.
Western Blotting
Western blot analysis of extracts from various cell lines using Di-Methyl Histone H3 (Lys36) Antibody #9758.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with synthetic methylated peptides (KLH-coupled) corresponding to residues surrounding Lys 4, 9, 27, 36 and 79 of human Histone H3. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Rabbit monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the amino terminus of histone H3 in which Lys4 is di-methylated.
Background
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases have been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1) and WD-40 domains (WDR5) (5-8). The recent discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic mark (9).
- Peterson, C.L. and Laniel, M.A. (2004) Curr. Biol. 14, R546-R551.
- Kubicek, S. et al. (2006) Ernst Schering Res. Found Workshop , 1-27.
- Lin, W. and Dent, S.Y. (2006) Curr. Opin. Genet. Dev. 16, 137-142.
- Lee, D.Y. et al. (2005) Endocr. Rev. 26, 147-170.
- Daniel, J.A. et al. (2005) Cell Cycle 4, 919-926.
- Shi, X. et al. (2006) Nature 442, 96-99.
- Wysocka, J. et al. (2006) Nature 442, 86-90.
- Wysocka, J. et al. (2005) Cell 121, 859-872.
- Trojer, P. and Reinberg, D. (2006) Cell 125, 213-217.
Application References
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