Product Pathways - Chromatin Regulation

Methyl-Histone H3 Antibody Sampler Kit #9847

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Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Source
Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb # 9725	40 microliters	W IP IHC-P IF-IC ChIP	H M R Mk	17	Rabbit
Di-Methyl-Histone H3 (Lys9) Antibody # 9753	40 microliters	W IP IHC-P IF-IC ChIP	H M R Mk (All)	17	Rabbit
Di-Methyl-Histone H3 (Lys27) Antibody # 9755	40 microliters	W IP IF-IC	H M R Mk	17	Rabbit
Di-Methyl-Histone H3 (Lys36) Antibody # 9758	40 microliters	W IP IF-IC	H M R Mk (All)	17	Rabbit
Di-Methyl-Histone H3 (Lys79) Antibody # 9757	40 microliters	W IP	H M R Mk (All)	17	Rabbit
Histone H3 Antibody # 9715	40 microliters	W IP IHC-P IF-IC	H M R Mk B (Dr)	17	Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074	100 microliters				Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat  All=All  Mk=Monkey  B=Bovine  Dr=Drosophila

Specificity / Sensitivity

All antibodies in the Methyl-Histone H3 Antibody Sampler Kit recognize histone H3 only when modified at the indicated site.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with synthetic methylated peptides (KLH-coupled) corresponding to residues surrounding Lys 4, 9, 27, 36 and 79 of human Histone H3. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Rabbit monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the amino terminus of histone H3 in which Lys4 is di-methylated.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases have been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1) and WD-40 domains (WDR5) (5-8). The recent discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic mark (9).

1. Peterson, C.L. and Laniel, M.A. (2004) Curr. Biol. 14, R546-R551.
2. Kubicek, S. et al. (2006) Ernst Schering Res. Found Workshop , 1-27.
3. Lin, W. and Dent, S.Y. (2006) Curr. Opin. Genet. Dev. 16, 137-142.
4. Lee, D.Y. et al. (2005) Endocr. Rev. 26, 147-170.
5. Daniel, J.A. et al. (2005) Cell Cycle 4, 919-926.
6. Shi, X. et al. (2006) Nature 442, 96-99.
7. Wysocka, J. et al. (2006) Nature 442, 86-90.
8. Wysocka, J. et al. (2005) Cell 121, 859-872.
9. Trojer, P. and Reinberg, D. (2006) Cell 125, 213-217.

Application References

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