Product Pathways - Chromatin Regulation / Epigenetics
Methyl-Histone H3 Antibody Sampler Kit #9847
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725||40 µl||W IP IHC-P IF-IC ChIP||H M R Mk||17||Rabbit IgG|
|Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb #4658||40 µl||W IP IF-IC ChIP||H M R Mk (Dm) (X) (Z) (B) (Pg) (Sc) (Ce)||17||Rabbit IgG|
|Di-Methyl-Histone H3 (Lys27) Antibody #9755||40 µl||W IP||H M R Mk||17||Rabbit|
|Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb #2901||40 µl||W IHC-P IF-IC||H M R Mk||17||Rabbit IgG|
|Di-Methyl-Histone H3 (Lys79) Antibody #9757||40 µl||W||H M R Mk||17||Rabbit|
|Histone H3 (D1H2) XP® Rabbit mAb #4499||40 µl||W IHC-P IF-IC||H M R Mk (Hm) (C) (Dm) (X) (Z) (B)||17||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Pg=Pig Sc=S. cerevisiae Ce=C. elegans
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
All antibodies in the Methyl-Histone H3 Antibody Sampler Kit recognize histone H3 only when modified at the indicated site.
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb #4658.
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725.
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys27) Antibody #9755.
Western blot analysis of extracts from various cell lines using Di-Methyl Histone H3 (Lys79) Antibody #9757.
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb #4499.
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb #2901.
The Methyl-Histone H3 Antibody Sampler Kit provides a fast and economical means of evaluating methylation sites on histone H3. The kit contains enough primary and secondary antibodies to perform four western blots.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic di-methylated peptides corresponding to residues surrounding Lys 4, 27 and 79 of human Histone H3. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic di-methylated peptides corresponding to residues surrounding Lys 9 and 36 of human Histone H3. Histone H3 (D1H2) XP Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human histone H3 protein.
The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).
- Peterson, C.L. and Laniel, M.A. (2004) Curr. Biol. 14, R546-R551.
- Kubicek, S. et al. (2006) Ernst Schering Res. Found Workshop, 1-27.
- Lin, W. and Dent, S.Y. (2006) Curr. Opin. Genet. Dev. 16, 137-142.
- Lee, D.Y. et al. (2005) Endocr. Rev. 26, 147-170.
- Daniel, J.A. et al. (2005) Cell Cycle 4, 919-926.
- Shi, X. et al. (2006) Nature 442, 96-99.
- Wysocka, J. et al. (2006) Nature 442, 86-90.
- Wysocka, J. et al. (2005) Cell 121, 859-872.
- Trojer, P. and Reinberg, D. (2006) Cell 125, 213-217.
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* Product-specific protocol.
- 9849 Phospho-Histone H3 (Mitotic Marker) Antibody Sampler Kit
- 9927 Acetyl-Histone H3 Antibody Sampler Kit
- 9928 Histone Deacetylase (HDAC) Antibody Sampler Kit
- 9933 Acetyl-Histone Antibody Sampler Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.