Cell Signaling Technology

Product Pathways - Transcription Factors

PAX8 Antibody #9857

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk (M) (R) Endogenous 48 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PAX8 Antibody recognizes endogenous levels of total PAX8 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Pax8 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PAX8 Antibody.

Background

Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).

PAX8 is involved in the development of thyroid follicular cells and the expression of thyroid-specific genes (5). Investigators have associated mutations in the PAX8 gene with thyroid dysgenesis, thyroid follicular carcinomas, and atypical follicular thyroid adenomas (6,7).

  1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
  2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
  3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
  4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.
  5. Kimura, S. (2011) J Thyroid Res 2011, 710213.
  6. Fagman, H. and Nilsson, M. (2011) J Mol Endocrinol 46, R33-42.
  7. Albarel, F. et al. (2012) Ann Endocrinol (Paris) , .

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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