Product Pathways - Cell Cycle / Checkpoint
Senescence beta-Galactosidase Staining Kit #9860
| Kit Includes | Quantity |
|---|---|
| Fixative | 15 milliliters |
| Staining Solution | 15 milliliters |
| Solution A | 1.5 milliliters |
| Solution B | 1.5 milliliters |
| X-Gal | 150 milligrams |
Description
The Senescence β-Galactosidase Staining Kit* is designed to histochemically detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.
Specificity / Sensitivity
The kit detects only β-galactosidase activity at pH 6 in cultured cells and tissue sections. β-galactosidase activity at pH 6 is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
IC-ABC
beta-galactosidase staining at pH 6 on normal WI38 cells at population doubling 29 (left) and senescent WI38 cells at population doubling 36 (right).
Background
Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.
- Goldstein, S. (1990) Science 249, 1129-1133.
- Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.
- Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.
- Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
- Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.
Application References
- Takaoka, M. et al. (2004) Ha-Ras(G12V) induces senescence in primary and immortalized human esophageal keratinocytes with p53 dysfunction. Oncogene 23, 6760-6768. This article references the use of Senescence beta-Galactosidase Staining Kit in the following applications: IF-P (paraffin)
- Baker, D. J. et al. (2004) BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat. Genetics 36, 744-749. This article references the use of Senescence beta-Galactosidase Staining Kit in the following applications: IHC-FL (floating/frozen)
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Companion Products
- 2372 β-Gal (14B7) Mouse mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
