Product Pathways - Cell Cycle / Checkpoint

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CDK Antibody Sampler Kit #9868

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
cdc2 Antibody #9112	40 µl	W	H M R	34	Rabbit
CDK2 (78B2) Rabbit mAb #2546	40 µl	W IP F	H M R Mk	33	Rabbit IgG
CDK4 (DCS156) Mouse mAb #2906	40 µl	W	H M	30	Mouse IgG1
CDK6 (DCS83) Mouse mAb #3136	40 µl	W	H M R	36	Mouse IgG1
CDK7 (MO1) Mouse mAb #2916	40 µl	W IP IHC-P	H R	40	Mouse IgG2b
CDK9 (C12F7) Rabbit mAb #2316	40 µl	W IP IHC-P IHC-F IF-IC F	H M R Hm Mk B Dg	42, 55	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat
Anti-mouse IgG, HRP-linked Antibody #7076	100 µl				Horse

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine  Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the CDK Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

Description

The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr15 of human cdc2; polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues of human CDK2 and recombinant human CDK7, amino acids 270-290 of recombinant human CDK4, amino acids 30-65 of human CDK6 and residues near the carboxy terminus of human CDK9.

Background

Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7).

1. Fattaey, A. and Booher, R.N. (1997) Prog Cell Cycle Res 3, 233-40.
2. Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6.
3. Jessus, C. and Ozon, R. (1995) Prog Cell Cycle Res 1, 215-28.
4. Kaldis, P. (1999) Cell Mol Life Sci 55, 284-96.
5. De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7.
6. Sherr, C.J. (1995) Trends Biochem Sci 20, 187-90.
7. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

2316:

Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

2546:

Flow, Immunoprecipitation, Western Blotting

2906:

Western Blotting

2916:

IHC / Paraffin, Immunoprecipitation, Western Blotting

3136:

Western Blotting

9112:

Western Blotting

Companion Products

• 7727 Biotinylated Protein Ladder Detection Pack
• 7076 Anti-mouse IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9869 Cyclin Antibody Sampler Kit

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For Research Use Only. Not For Use In Diagnostic Procedures.