Product Pathways - Cell Cycle / Checkpoint
CDK Antibody Sampler Kit #9868
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|cdc2 Antibody #9112||40 µl||W||H M R||34||Rabbit|
|CDK2 (78B2) Rabbit mAb #2546||40 µl||W IP F||H M R Mk||33||Rabbit IgG|
|CDK4 (DCS156) Mouse mAb #2906||40 µl||W||H M||30||Mouse IgG1|
|CDK6 (DCS83) Mouse mAb #3136||40 µl||W||H M R||36||Mouse IgG1|
|CDK7 (MO1) Mouse mAb #2916||40 µl||W IP IHC-P||H R||40||Mouse IgG2b|
|CDK9 (C12F7) Rabbit mAb #2316||40 µl||W IP IHC-P IHC-F IF-IC F||H M R Hm Mk B Dg||42, 55||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-mouse IgG, HRP-linked Antibody #7076||100 µl||Horse|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey B=Bovine Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the CDK Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using CDK9 (C12F7) Rabbit mAb #2316.
Western blot analysis of extracts from various cell types using CDK2 (78B2) Rabbit mAb #2546.
Western blot analysis of extracts from SK-N-MC and mIMCD-3 cells using CDK4 (DCS156) Mouse mAb #2906.
Western blot analysis of extracts from SK-N-MC and C6 cells using CDK7 (MO1) Mouse mAb #2916.
Western blot analysis of extracts from HeLa, mIMCD-3 and C6 cells using CDK6 (DCS83) Mouse mAb #3136.
Western blot analysis of extracts from Saos-2 cells, either untreated or treated with hydroxyurea or nocodazole, using Phospho-cdc2 (Tyr15) Antibody #9111 (upper) and cdc2 Antibody #9112 (lower).
The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr15 of human cdc2; polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues of human CDK2 and recombinant human CDK7, amino acids 270-290 of recombinant human CDK4, amino acids 30-65 of human CDK6 and residues near the carboxy terminus of human CDK9.
Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7).
- Fattaey, A. and Booher, R.N. (1997) Prog Cell Cycle Res 3, 233-40.
- Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6.
- Jessus, C. and Ozon, R. (1995) Prog Cell Cycle Res 1, 215-28.
- Kaldis, P. (1999) Cell Mol Life Sci 55, 284-96.
- De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7.
- Sherr, C.J. (1995) Trends Biochem Sci 20, 187-90.
- Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
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For Research Use Only. Not For Use In Diagnostic Procedures.